I just solve!!
In Class JpaTransactionManager say:
Note: To be able to register a DataSource's Connection for plain JDBC code, this instance needs to be aware of the DataSource (setDataSource(javax.sql.DataSource)). The given DataSource should obviously match the one used by the given EntityManagerFactory. This transaction manager will autodetect the DataSource used as known connection factory of the EntityManagerFactory, so you usually don't need to explicitly specify the "dataSource" property.
and you need create a special bean to mix transaction like this:
<bean id="jpaDialect" class="org.springframework.orm.jpa.vendor.HibernateJpaDialect"/>
<bean id="transacaoJPA" class="org.springframework.orm.jpa.JpaTransactionManager">
<property name="entityManagerFactory" ref="emf"/>
<property name="dataSource" ref="poolBean"/>
<property name="jpaDialect" ref="jpaDialect"/>
</bean>
See yaa