Depends on what you are trying to do. If the connection is closed, then the next connection you open will likely have autoCommit set to 'true'. In which case you'll have to set it to false upon each opening of a new connection.
However,
you should only need to set autoCommit back to true if the connection is still open and you're doing additional processing with it where you want it to autoCommit. Can't think of any examples personally since I pretty much set it to autoCommit false and leave it that way.